Nonspecific Interaction of Streptavidin with Urease-Conjugated Antibodies

摘要

Streptavidin (SA) biotin interactions are used in many aspects of bioanalysis andmolecular biology. The biosensor of the Bio-Chemical Detector (BCD) employs SA biotin-mediated filtration-capture onto nitrocellulose membranes in its immunoassay procedures. During the development of immunoassays for the BCD biosensor, systematic variations in the background and the sensitivity were observed. The results presented in this work indicated that the cause of these variations was nonspecific interaction of SA with one of the components of the reagent mixture, a urease-conjugated antibody. The variations could be diminished by allowing the reagents to stand at room temperature for two to three hours prior to use.