Light-Addressable Potentiometric (LAP) Sensor Assay of Newcastle Disease Virus

摘要

A sandwich immunoassay for Newcastle Disease Virus (NDV) was developed usingstreptavidin-biotin mediated filtration capture and silicon-based semiconductor detection. The assays were carried out in one step whereby the immunoreagents and the antigen (NDV) were mixed together and incubated. The pH sensing capability of the detector, a light addressable potentiometric (LAP) sensor, was employed to detect the presence of immobilized urease-conjugated antibodies. Lower limits of detection (LOD) of the assay were determined as a function of incubation time. The LOD were approximately 27, 15 and 3 ng per well for 1, 5 and 60 minute incubations, respectively. The techniques described here have the potential to be adapted to an automated LAP sensor for use in the Biochemical Detector program.