Polymyxin B(PMB)-Specific Monoclonal Antibodies

摘要

As has been reported earlier, PMB conjugated to either Bovine Serum Albumin (BSA)or Keyhole Limpet Hemocyanin (KLH) were very immunogenic in both rabbits and Balb/c mice. The latter was not as immunogenic as the PMB-BSA conjugate largely because of it insoluble state. Both preparations have lost significant antibacterial properties suggesting that a significant number of the diaminobutyric acids were substituted by the conjugation process. The conjugated PMB preparations were still toxic to mice when given intravenously in 2 ug quantity. Multiple intraperitoneal or subcutaneous injections of 100 ug of PMB-BSA in the presence of Freund's adjuvants stimulated the production of high levels of PMB-specific antibodies in Balb-c mice.