Structure/Function Studies of T-2 Mycotoxin with a Monoclonal Antibody

摘要

A BALB/C murine monoclonal antibody (mAb) specific for the trichothecenemycotoxin T-2 was previously generated. The anti-T-2 antibody, designated HDII, can detect T-2 in the nanogram range employing an enzyme-linked immuno-sorbent assay. The HDII antibody at a concentration of 1 ug/ml completely protected against the T-2-induced cytotoxicity of the human epidermoid carcinoma cell lines Hep-2 and KB. Fine specificity analysis was performed using 10 structurally related T-2 metabolites to inhibit the specific binding of HDII to T-2 mycotoxin. The results suggest a binding specificity of the protective HDl 1 antibody for the bulky hydrophobic alkyl side chain at position R5 and the acetyl groups at positions R2 and R3 of the T-2 mycotoxin molecule. HD1 1 anti-T-2 mAb, which bound to the T-2 metabolite acetyl T-2 (with a substituted acetyl group at R1 position), efficiently.