Awake Porcine Model of Intraperitoneal Sepsis and Altered Oxygen Utilization

摘要

We have characterized an awake swine model of septic shock. Hemodynamic, serumchemistry, and oxygen metabolism parameters were compared between eight septic and five sham animals. Eight male Yucatan miniature swine, weighing 20-28 kg, were anesthetized and catheters were placed in the pulmonary artery, external jugular, and the carotid artery. On day 2, 1.1-4.0 X 1010 cfu Escherichia coli/kg were administered via an intraperitoneal catheter. Hemodynamic parameters were monitored hourly for 6 hours in awake animals. The animals were then placed back into the animal holding facility for clinical observation until the 24 hour post infusion measurements were taken. Septic animals were initially hypodynamic, with a decrease in cardiac index (Cl) from a baseline value of 152.8 to 87.9 ml/kg/min and an increased systemic vascular resistance index (SVRI) from a control value of 48.1 to 65.0 dynes sec cm-5/kg.