Research in Drug Development against Viral Diseases of Military Importance(Biological Testing)

摘要

The purpose of this program is to evaluate the efficacy of candidate antiviralcompounds against a spectrum of viruses of military importance. This program involves (a) primary testing of chemical compounds and natural products for antiviral efficacy in vitro using CPE-inhibition, plaque-reduction, and MTT assays, (b) primary testing of compounds for antiviral efficacy in vivo in animal model systems, and (c) secondary evaluation of the active candidate antiviral compounds. The target viruses for in vitro testing are Vaccinia Virus (VV), Adenovirus (AD2), Vesicular Stomatitis Virus (VSV), Punta Toro Virus (PT), Sandfly Fever Virus (SF), Yellow Fever Virus (YF), Venezuelan Equine Encephalomyelitis Virus (VE), Japanese Encephalitis Virus (JE), Pichinde Virus (PIC), and Hantaan Virus (HTN). The in vivo systems are Pichinde Virus infection of hamsters, Venezuelan Equine Encephalomyelitis Virus, Japanese Encephalitis Virus and Vaccina Virus infection of mice.