Tissue-Specific Expression of epsilon and alpha Messenger Ribonucleic Acid inAllergy-Prone C3H Mice

摘要

Intraperitoneal inoculation of Rift Valley fever virus (RVFV) in C3H substrainsof mice resulted in acute respiratory distress of all C3H/HeJ mice upon repeat exposure on day 14. In contrast, no C3H/HeN mice were affected by reexposure to RVFV. This phenomenon was never observed until C3H/HeJ mice were substituted for other strains because of increased immunoglobulin A (IgA) responses. We examined immunoglobulin heavy chain gene expression by in situ hybridization in sections and by Northern blot analysis of extracted tissue ribonucleic acid (RNA) from control and RVFV-immunized mice. Adult female C3H/HeJ, C3H/HeN (OuJ), mice were vaccinated intraperitoneally (i.p.), subcutaneously (s.c.), and intranasally (i.n.). Mice were killed via CO2 exposure. Blood IgA and IgE levels were determined by ELISA. Rift Valley fever virus vaccine (IND 365) was prepared from Entebbe strain virus grown on fetal rhesus lung cells and was inactivated with formalin. Peripheral, mediastinal, and mesenteric lymph nodes (LNs), spleen, intestines, and lungs were removed and immediately frozen in liquid nitrogen (organs from each group were pooled). Ribonucleic acid was isolated from frozen mouse tissues by the method of Chirgwin et al. Reprints