Identifying the Mechanism(s) Responsible for the Translational Regulation of the Stress Signaling Kinase MKK4.

摘要

Findings reported herein detail our progress on the development of prostate cancer cell line reagents, which are critical to our proposed studies of micro RNA-mediated regulation of the MKK4 protein. Since our last progress report we have focused on studies in Specific Aim 1, Major Task 2, subtask 1d and 1e. Specifically, building on our previous findings, we now have determined the growth kinetics and metastatic site preference of CWR22Rv1-luc2 cells after intracardiac injection using both IVIS imaging and histology. We have also made significant progress in our efforts to develop bone-seeking cell lines from CWR22Rv1-luc2 cells. Thus far, our effort to develop bone-seeking sublines of the CWR22Rv1 cell line is on track. To be frank, the work in progressing at a faster pace and with cleaner results that we expected. Thus, we are in an excellent position to address the work proposed for YR5 of our EWOF.