Human Immune Response to Dengue Infections.

摘要

We have mapped the epitopes recognized by the serotype-cross-reactive clone JK3 which is located within amino acids (a.a.) 251-165 of NS3. The epitopes recognized by the serotype-cross-active clone JK32 and the flavivirus cross-reactive clone JK43 are located within a.a. 1-452 and a.a.1-183 of NS3, respectively. We also established 16 dengue virus-specific CD8+ CD4- T cell clones. The clone 2.8 recognized dengue virus types 2 and 4, and the clone 2.9 recognize four serotypes of dengue viruses. The clones 2.8 and 2.9 recognized NS3 protein, and the epitope recognized by the clone 2.8 is located within a.a. 453-618. These results indicate that NS3 contains multiple dominant T cell epitopes recognized by CD4+ and CD8+ human CTL. To analyze the mechanisms of antibody-dependent enhancement, we prepared bispecific antibodies.