Localization of Immunogenic Domains in the Human Immunodeficiency Virus Type 2Envelope

摘要

Highly immunogenic domains have not yet been defined in the extracellular proteinof the human immunodeficiency virus type 2 (HIV-2) envelope. In this study, six contiguous segments covering the entire HIV-2 sub st envelope were amplified and cloned into a bacterial expression vector to localize the relative immunogenic reactivity of different regions of the molecule by Western blot (immunoblot) analysis. Our results demonstrate that the extracellular protein of the HIV-2 envelope contains highly immunogenic epitopes with potential value as type-specific markers for HIV-2 infection. Reprint, AIDS, HIV, Vaccines, Biotechnology, RAD I.