Ultraviolet Irradiation of DNA Complexed with Alpha/Beta-Type Small, Acid-SolubleProteins from Spores of Bacillus or Clostridium Species Makes Spore Photoproduct But Not Thymine Dimers

摘要

UV irradiation of complexes of DNA and an alpha/beta type small, acid solubleprotein (SASP) from Bacillus subtilis spores gave decreasing amounts of pyrimidine dimers and increasing amounts of spore photoproduct as the SASP/DNA ratio was increased. The yields of pyrimidine dimers and spore photoproduct were < 0.2% and 8% of total thymine, respectively, when DNA saturated with SASP was irradiated at 254 nm with 30 kJ/m2; in the absence of SASP the yields were