Role of Conserved gp41 Cysteine Residues in the Processing of HumanImmunodeficiency Virus Envelope Precursor and Viral Infectivity

摘要

All animal retroviruses whose nucleotide sequences have been determined containtwo or three closely spaced cysteine residues in the extracellular domain of the env-encoded transmembrane protein. Using human immunodeficiency virus type 1 gp41 as a working model, the functional significance of these highly conserved cysteines was investigated. We report here that substituting the two conserved cysteine residues in this domain of gp41 with glycine residues resulted in the loss of viral infectivity, which could be attributed to severe impairment in the processing of gp160 precursor to gp120. Reprint, AIDS, HIV, Vaccines, Biotechnology, RAD I.