Method for the Quantitative Analysis and Standardization of Interleukin-1Bioactivity

摘要

Interleukin 1 (IL-1) is a polypeptide mediator produced by activated macrophagesand monocytes. It has a broad spectrum of biological functions in immunological and inflammatory reactions (as reviewed in 5, 12, 10). Two distinct species, have been identified and molecularly cloned. Although there is a limited homology between the two species, they mediate the same biological events and appear to bind to the same receptors on target cells. Operationally, IL-1 is defined by its ability to potentiate the proliferation of murine thymocytes stimulated by suboptimal doses of mitogens in the thymocyte proliferation assay. A variety of bioassays of the lymphocyte proliferative effects of IL-1 have been developed by using a helper T-cell line (D 10.G4.1). Reprints.