Development of Carcinogenesis Bioassay Models: Response of Small Fish Species toVarious Classes of Carcinogens

摘要

Studies were conducted related to the development of small fish carcinogenesisbioassay models using the medaka (Oryzias latipes) and the guppy (Poecilia reticulata). The specific aim of the project was to identify certain classes of carcinogens that would induce neoplastic lesions in those model species. Bioassays were conducted on the following compounds: the halogenated hydrocarbons 1,1,2,2-tetrachlorothane (TeCE), chlorodibromethane (CDBM), and vinylidene chloride (VDC), the heavy metal cadmium (Cd), the aromatic amines 2-acetylaminofluorene (AAF) and 4-aminobiphenyl (ABP), the nitrogen-substituted polyaromatic hydrocarbon dibenzocarbazole (DBC), the industrial product acrylonitrile (AN) and the pharmaceutical methapyrilene (MP). Bioassays were conducted TeCE, CDBM, VDC, Cd, AAF, ABP, AN, and MP on medaka and guppy and DBC on medaka only. Mechanisms of ethylene dibromide metabolism were examined in medaka and of acetylaminofluorene in medaka and guppy. Other carcinogen bioassay factors such as the occurrence of background and rare carcinogen-induced tumors were examined in both species.