Screening of Organophosphorus Acid Anhydrases from Different Sources by WesternBlot Analysis

摘要

Enzymes that are capable of degrading organophosphorous compounds (e.g.,acetylcholinesterase inhibitors, pesticides, and G-type agents) have been found in both prokaryotic and eukaryotic organisms. These enzymes, referred to as organophosphorous acid anhydrases (OPA anhydrases), offer considerable potential for use in chemical agent decontamination systems. One of these enzymes, OPAA-2 from halophilic JD6.5, has recently been purified to homogeneity. The polyclonal and monoclonal antibodies against OPAA-2 have been prepared and shown to react specifically with this enzyme. Using this monoclonal antibody as a probe to screen OPA anhydrases from various sources in Western blot analysis, it was found that microorganisms with high levels of OPA anhydrase can be recognized by their unique binding ability.