Functional Proteomics to Identify Moderators of CD8+ T-Cell Function in Melanoma.

摘要

In the current funding period we have performed several rounds of phage-screens to select clones that differentially bind to either tumor infiltrating cytotoxic (CD8+) lymphocytes, activated CD8+ lymphocytes from the spleen, or un-activated na ve CD8+ T cells. We have developed a high-throughput flow cytometric approach that allows us to screen the specificity of several phage clones for each of these CD8+ populations. We have identified phage that selectively bind to na ve CD8+ T cells and those that bind to both tumor- infiltrating and effector CD8+ T cells, serving as a proof of principle for the strategy. We are making modifications to: (1) the phage libraries that are screened; and (2) the methods by which phage clones are selected for screening in order to increase the repertoire of phage that bind to TIL selectively.