Serum Antibody Responses to Oral Microorganisms in Nonhuman Primates

摘要

Porphyromonas gingivalis and Prevotella intermedia have been associated withperiodontitis in humans and nonhuman primates (NhP). this investigation characterized serum antibody levels, isotypes and subclass distribution and specificity to P. gingivalis, P. intermedia and Bacteroides fragilis prior to, and after immunization with these bacteria and during ligature-induced periodontitis. Serum from 20 adult, female cynomolgus monkeys was obtained at baseline, after 3 intramuscular injections with 10 to the 9th power bacteria emulsified in incomplete Freund's adjuvant (IFA) or with IFA, and during 30 wks. of ligature placement. IgG, IgM and IgA isotypes and IgG1-4 subclass antibody were assessed in an ELISA. No detectable changes were noted in IgG/M/A antibody to P. gingivalis or P. intermedia in any group following ligation. These results demonstrate the ability to induce a highly specific antibody response in NhP following immunization with oral microorganisms. While all isotypes were elicited, only the IgG antibody was maintained through the ligation interval. The natural and induced IgG response to the oral bacteria was primarily IgG1. Due to the extensive capsule that has been described on these microorganisms, this type of IgG response may be ineffective in protecting from disease due to its inability to opsonize these microorganisms.