Immunoassay of Mouse Immunoglobulin G by a Light-Addressable PotentiometricSensor

摘要

A sensitive enzyme immunoassay for the quantitation of mouse immunoglobulin G(mIgG) was developed using a light-addressable potentiometric (LAP) sensor as the detection system. The assay was carried out on nitrocellulose membrane filters and used sandwich-format one-step incubations of antigens and antibodies. In addition, the assay employed a high affinity biotin-streptavidin interaction to capture the immunocomplexes onto nitrocellulose membranes, and a urease-conjugated anti-mouse IgF detector antibody.