IGG Subclass and Isotype Specific Immunoglobulin Responses to Lassa Fever andVenezuelan Equine Encephalomyelitis: Natural Infection and Immunization

摘要

Venezuelan Equine Encephalitis(VEE) specific immunoglobulin responses to the twovaccines, TC-83 (a live-attenuated vaccine) and C-84 (a formulin inactivated vaccine derived from the TC-83 strain) were evaluated using an antigen and isotype specific enzyme-linked immunoabsorbent assay (ELISA). The VEE-specific ELISA was developed and standardized using selected sera from individuals previously immunized. Initial experiments demonstrated that non-specific binding in the IgG, G2, A and M analysis was a significant problem in data interpretation. Preparation of a negative control (tissue culture media derived) antigen was utilized to further standardize assays. All assays were standardized in methodology and in relation to a uniform reference curve, where available. Initial screening of 28 SERA demonstrated the presence of predominantly VEE-specific Gl and G3 subclasses, IgA and M response were also demonstrated in the series of subjects who received the TC-83 vaccine as well as in response to a C-84 boost vaccine.