Quantifying the Kinetic Processes Associated with HIV Infection of Target Cells (AIDS).

摘要

The primary purpose of this project was to develop mathematical models of HIV infection that simulated kinetic processes taking place in viral infectivity assays. The secondary purpose was to develop a series of decisive experimental tests of these kinetic models. The overall goal was to determine whether kinetic modeling improves the quality and reliability of data derived from quantitative infectivity assays, and to see whether the data from model-directed assays lends further insight into in vivo infectious processes. We believe that the specific objectives of this project have been achieved. The findings of this study have practical applications to the standardization of infectivity, assays, the testing of therapeutic agents binding to viral gpl2O and cellular CD4, and the evaluation of HIV vaccines. We also see improvements in the ability to extrapolate from assays conditions in vitro to physiologic conditions in vivo.