Acute Delayed Neurotoxicity Evaluation of Two Jet Engine Oils using a Modified Navy and EPA Protocol.

摘要

This study was initiated with three objectives. The first was to determine if either of two jet engine oils had the potential to produce delayed neuropathy. The major component of each oil is a mixture of hydrocarbon-based esters. One formulation contained 3% tricresylphosphate (TCP) isomer additive, whereas the second contained 3% of the ortho derivative of tricresylphosphate (TOTP), a known neurotoxicant. A second objective was to determine if the Navy repeated-high-dose of 420 mg/kg/day was sufficiently sensitive to determine neurotoxicity. The last was to compare the results of the former Environmental Protection Agency (EPA) limit test of 5 g/kg with the new standard of 2 g/kg. The assays performed indicated that the jet engine oil containing TOTP produced delayed neuropathy, whereas the jet engine oil containing TCPI did not. Repeated dose levels greater than 420 mg/kg/day (1000 and 2000 mg/kg/day) produced organophosphorus-induced delayed neuropathy (OPIDN), whereas hens dosed at 420 mg/kg/day were asymptomatic. No potential for OPIDN was indicated in hens treated with a single dose of 2 g/kg, but hens dosed at the previous EPA limit dose had significant brain neurotoxic esterase (NTE) inhibition and axonopathy by 30 days posttreatment. Axonopathy, Neurotoxicity, Chickens, Tricresylphosphate, Jet engine oil formulation, Tri-o-tolyl Phosphate, Neurotoxic esterase.