Combined Effects of Hyperbaric Oxygen and Antimicrobials in a Model of Gas Gangrene.

摘要

The efficacy of hyperbaric oxygen (HBO) alone and in combination with several antimicrobial agents was evaluated in a lethal model of gas gangrene in mice. Myonecrosis was induced by injecting -1079 washed C. perfringens into the right upper thigh'-muscle of mice; Immediately following bacterial inoculation, penicillin, imipenem, clindamycin or metronidazole were administered via, intraperitoneal injection. HBO treatments PO2 = 301 kPa for 90 min, B.I.D. X days began immediately after antimicrobial injections. Survival of mice was monitored for 72 h. Mice treated with clindamycin or metronidazole survived significantly longer-than mice treated with penicillin G or imipenem (P - 0.05). HBO alone did not prolong time-to-death in control animals as compared to air. Also, HBO did not potentiate the efficacies of any of the antimicrobials tested in this model. Hyperbaric Oxygen, Clostridial myonecrosis, Alpha toxin, antimicrobials.