Determination of 5-Bromo-2'-Deoxyuridine (BrdU) in Well Water by High Performance Liquid Chromatography (HPLC).

摘要

A liquid chromatographic method has been developed for the analysis of 5-Br deoxyuridine (BrdU) in well water. The samples are injected directly onto a high performance liquid chromatograph (HPLC) without further sample preparation. Separation of BrdU from possible interferences found in well water was achieved by using a C-18 column and a mobile phase containing water and methanol. A programmable ultraviolet (UV) detector was used to monitor the effluent for BrdU. HPLC provides a rapid and reproducible method for the analysis of BrdU. High Performance Liquid Chromatograph (HPLC), 5-Bromo-2'deoxyuridine (BrdU), Ultraviolet (UV).