Glycosphingolipids as Putative Receptors of Staphylococcal Enterotoxin B in HumanKidney Proximal Tubular Cells

摘要

We have investigated the binding of 125 I staphylococcal enterotoxin-B (SEB) toglycosphingolipids (GSL) from cultured human kidney proximal tubular cells employing GSL overlay technique and microplate quantitative assay. Structural studies of the putative GSL receptor for SEB was performed employing high performance liquid chromatography (HPLC), gas liquid chromatography, mass spectrometry (GC-MS) of Trimethylsilyl derivatives of methylglycosides, fatty acids and sphingosines. Permethylation analysis of sugars was carried out employing GC-MS. Our results are: 1. Out of twelve GSL present in human kidney, only a diglycosyl ceramide specifically bound to SEB. 2. The chemical composition of the putative receptor: carbohydrates, glucose, galatose, sphingosines, dl8:2, d23:0, fatty acids, C16:0, C18:1. RA 1, Glycosphingolipids, Receptors, Kidney proximal tubular cells, High performance thin layer chromatography.