Application of High-Performance Liquid Chromatography Assay for MonitoringKinetics of Interconversions of Stereoisomers of Thymidine Glycol

摘要

A reverse-phase high-performance liquid chromatography methodology was validatedfor rapid, sensitive and simultaneous analysis of all stereoisomers of thymidine glycol. The procedure involved direct injection of the samples on a microbore C-18 reverse-phase column with ultraviolet detection at 220 nm wavelength. The lower limits of detection for all thymidine glycol stereoisomers were close to 2.5 pmole under optimized conditions of detection and were linear up to at least 5000 pmole. The mobile phase consisted of 3% acetonitrile in water (v/v). The procedure allowed qualitative as well as quantitative measurements of pH and temperature-dependent interconversions of isomers of thymidine glycol directly (underivatized) from aqueous solution.