Genetic Methods for Rapid Gene Localization in Hyperthermophilic Eubacteria.

摘要

We initiated investigations along several lines to develop genetic methods of analyses with Thermotoga neapolitana. (1) We investigated the conditions necessary for optimal growth of T. neapolitana on solid media under anaerobic conditions. (2) We attempted to isolate mutants to provide genetic markers for the development of gene transfer methods. (3) We explored a number of methods to introduce DNA into T. neapolitana. (4) A cryptic Thermotoga plasmid was discovered and its potential as a cloning vector was investigated. (5) A cosmid library and large chromosomal fragments will be used to located and map genes.