Prostaglandin E2, Interleukin-1 Alpha, and Potassium Release from ArtificialHuman Skin After Freeze-Thaw Injury

摘要

Living skin equivalent (LSE) was used to identify nonvascular aspects of freeze-thaw injury to human skin cells. Pairs of transwell cell culture inserts, containing LSE, were washed twice for 30 min in maintenance media at 37 deg C in a CO2 incubator and placed in two wells of a six-well assay tray (containing assay media). One specimen was maintained at room temperature. The other was cooled at 1 deg C/min to -15 deg C and rapidly rewarmed. Both were incubated for 24 h on fresh maintenance media at 37 deg C in a CO2 incubator. This process was repeated producing 12 per group. Prostaglandin E2 (PGE2), interleukin-1 alpha (IL-