Task 89-07: Evaluation of the In Vitro Efficacy of Candidate Pretreatment andTreatment (PT) Compounds Against Vesicants and Nerve Agents

摘要

MREF Task 89-07 encompassed four vesicant assays and four nerve agent assays. Thefour vesicant assays evaluated the candidate P and T compound solubility limitations, direct cytotoxic effects, efficacy against HD-induced cellular nicotinamide adenine dinucleotide (NAD+) depletion, and efficacy against HD-induced cytotoxicity. Normal human epidermal cells (NHEKs) were used to evaluate candidate PT compound efficacy against HD-induced NAD+ depletion, and peripheral blood mononuclear leukocytes (PBMC) were used in direct cytotoxicity and HD-induced cytotoxicity assays. The four nerve agent assays assessed candidate PT compound direct inhibitory effects on acetylcholinesterase (AChE) activity,