Microbial Degradation of Poly-B-esters: a Mechanistic Study, Cellulose AcetateBiodegradability

摘要

In this Final Report, work carried out under ARO grant C-DAAL03-G-0111 isdescribed. The investigations performed include the following: (1) isolation, purification and characterization of a poly(3-hydroxybutyrate) depolymerase enzyme from Penicillium funiculosum, (2) determination that the depolymerase is a serine esterase, (3) study of the effect of polymer stereochemistry and crystalline order in a semi-crystalline polymer film substrate on enzyme specificity and activity, (3) isolation, purification and characterization of cellulose acetate degrading microorganisms and (4) determination of the biodegradability of cellulose acetate with degrees of substitution up to 2.5 under aerobic thermophilic conditions. Poly(3-hydroxybutyrate) biodegradation, Poly(3-hydroxybutyrate) depolymerase enzyme, Depolymerase from Penicillium funiculosum, Cellulose acetate degrading microorganisms, Composting polymer biodegradable.