Photoprotein Aequorin Structure Determination by NMR Spectroscopy.

摘要

The nine month grant period was used to acquire experience making sequence specific assignments of proteins using homo- and heteronuclear 2D-NMR. Assignments were made for approximately 95% of the 1H and 15N atoms of Bacillus subtilis HPr phosphorylated at Serine 46. Attempts to carry out preliminary homonuclear 2D-NMR studies on r-aequorin were unsuccessful due to serious solubility problems encountered with a lyophilized sample of the photoprotein which had been charged with benzyl coelenterazine. Arrangements are underway to obtain a suitable NMR sample for preliminary analysis. A brief discussion of the methodology that will be required to obtain a solution structure of r-aequorin is included.... Aequorin, Bioluminescence, Nuclear magnetic resonance, 3D Structure.