Antigen and Genome Detection of Arenavirus, Bunyavirus, and Filovirus Infections

摘要

The specific aims of this project were to develop detection systems forrecognizing and quantitating antigens and genomes of hemorrhagic fever viruses, and to use these detection systems to investigate the pathogenesis of these viruses in animal models. These were to be accomplished using existing antibodies and nucleic acid probes supplied by the MRDC as well as virus infected tissues or cell cultures. Pichinde virus, an arenavirus non-pathogenic to humans, is a New World arenavirus. Previous reports indicate similarities between Pichinde infection in guinea pigs and Lassa fever infections in guinea pigs and monkeys. We examined Pichinde-infected Strain 13 guinea pigs to gain some insight into the pathogenesis of these viruses Gene probes, Diagnosis, RAI, BD, Arenaviruses, Bunyaviruses, Filoviruses.