Lifetime Fluorescence and Raman Imaging for Detection of Wound Failure and Heterotopic Ossification.

摘要

The aim of this effort was to develop Raman and lifetime fluorescence imaging systems to assess failed wounds. The goal of these studies was to develop novel contrast mechanisms based on tissue pathologies which allow surgeons to identify wounds at risk for failure based on classifiers identified with these imaging systems. Our collaborative team built and characterized novel video rate imaging systems based on these technologies as a first step towards enabling early and accurate detection of failed wounds. Soldiers wounded in combat present with acute, traumatic wounds resulting from blasts, high speed projectiles, or intense burns. These injuries exhibit complicated healing responses and often fail to heal despite the application of advanced treatments [1-3]. Long term failure facilitates increased risk of infection and can lead to permanent disabilities or death. Current treatment methods include regular debridement of wounds, prophylactic antibiotics, special dressings, and wound drainage systems [1, 4]. Histopathologic testing of excised tissue from wounds for abnormal collagens, inflammatory cytokines, and phosphates have provided insight into the pathophysiology of wounds [1, 5, 6]. However, these are research techniques and due to their expense cannot be done with sufficient frequency to predict wound failure. We hypothesize that systems based on Raman and lifetime fluorescence imaging can give early warning of possible wound failure and provide additional information that will refine the treatment plan and improve wound healing prognoses.