Tri-Elisa for Simultaneous Analysis of Three Analytes

摘要

The goal of this research effort is to develop a three-enzyme ELISA system inwhich three malarial analytes can be quantitated concurrently from a single biological sample. During the Phase I effort, we were able to develop a non-quantitative tri-ELISA that was capable of differentiating between three Plasmodium antigens/antibodies previously identified and optimized by Dr. Robert Wirtz at the Walter Reed Army Institute of Research. During this Phase II effort, we expect to improve and optimize the system, as well as provide both antibody pre-coated microplates and final kits for analysis by Dr. Wirtz. The resulting system will provide an efficient and potentially productive approach for improving an existing diagnostic assay for malarial sporozoites. The assay can be used by the World Health Organization for epidemiological studies designed to monitor, and through preventative mechanisms, potentially eradicate a disease which currently threatens greater than 56% of the world's population.