Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments

摘要

A modification to allow direct testing of soils and sediments has been made tothe standard immunoassay procedure. The modified procedure eliminates the need for extraction prior to ELISA testing. The new method has been successfully tested using 2,4-dinitrobenzene sulfonate as the model pollutant and crushed brick and sand as model soil matrices. The modified ELISA is very sensitive and easily distinguishes between contamination levels. Monoclonal antibodies were produced from antigens created from 4-chloroaniline, 2,4-dichloroaniline, and 3,4-dichloroaniline. One cell line of the anti-4-chloroaniline antibodies reacts to 4-chloroaniline, 2,4,5-trichloraniline, and 2,4,5-trichlorophenol. Immunoassay, Sediment testing, ELISA.