Electrical Communication between Glucose Oxidase and Electrodes Based onPoly(Vinyl Imidazole) Complex of Os(bpy)2Cl2

摘要

Glucose oxidase was electrically wired to glassy carbon electrodes by redoxhydrogels based on poly(N-vinyl imidazole) (PVI) complexed with Os(bpY)2C12(PVI-Os). The hydrogels were formed by crosslinking PVI-Os and glucose oxidase with poly(ethylene glycol) diglycidyl ether (PEGDGE). Glucose was electrooxidized on the PVI-Os based wired enzyme electrodes at 350 mV vs SCE. The dependence of the electrooxidation current on pH, ionic strength, film thickness, weight fraction of the enzyme in the redox hydrogel and oxygen partial pressure for electrodes rotating at 1000 rpm is described.