Neuroprotective k-Oploid Cl-977 Alters Glutamate-Induced Calcium Signaling Invitro

摘要

The effect of the neuroprotective kappa opioid CI-977 on glutamate (GLU)-stimulated calcium signalling was studied in individual primary rat cortical neurons. Using laser scanning microscopy and the fluorescent calcium probe fluo-3, both the sustained and biphasic intracellular calcium concentration (Ca(2+)) changes induced by GLU (20-40 microns) were altered by CI-977 (25-100 nM), thereby shifting the neuronal population response from unbuffered to buffered patterns of (Ca(2+1)) flux. This effect was consistent with the previously demonstrated neuroprotective action of CI-977 against glutamate toxicity in vitro. The effect of CI-977 in altering GLU-induced (Ca(2+)) signalling was attenuated by naloxone, consistent with a neuroprotective action of CI-977 at oploid receptors, presumably of the kappa subtype.