Detergent Pretreatment of Solid Phase Globular Proteins in ELISA's. EnhancedAntigenicity and Subsequent Sensitivity

摘要

Methods for pretreatment and rejuvenation of preimmobilized globular proteinsused in immunodiagnostics were investigated using reagents routinely used in ELISA's. Rabbit and goat gamma globulins, functioning as antigens, and antibodies on non-covalent, and covalent solid surfaces, were monitored for detergent mediated desorption, denaturation, non-specific binding and altered antigenicity.