Identification of Sulfate-Reducing Bacteria in Saline Oil Field and Navy PlatformSamples by Reverse Sample Genome Probing

摘要

Reverse sample genome probing (RSGP) allows fingerprinting of environmentalmicrobial communities in a single hybridization step. Application of this method to the analysis of microbial communities present on installed corrosion coupons in oil treaters in saline oil fields in Alberta indicated that of 20 different bacterial standards (defined as bacteria with little or no genomic crosshybridization), including 15 SRB only a few Desulfovibrio species were highly enhanced in the metal-associated biofiim, suggesting that these may be particularly active in Promoting metal corrosion. RSGP analysis of nine Navy platform isolates with master filters Containing genomes of SRB from saline oil fields indicated the presence of significant amounts of two of these 'corrosive' Desulfovibrio species in five of the nine platform isolates. The presence of these two Desulfovibrio Species may correlate with the potential of the platform isolates to cause microbially influenced metal corrosion.