Determination of Fifty Percent Inhibitory Concentrations (IC50) of AntimalarialDrugs Against Plasmodium Falciparum Parasites in a Serum-Free Medium

摘要

Plasmodium falciparum chemosensitivity to the various antimalarial drugs ispresently determined in the laboratory by setting up multiple microcultures of the parasite and estimating the amount of growth inhibition caused by known concentrations of drug. Parasite growth inhibition is assessed either by microscopy. radiolabeled substrate uptake or calorimetrically. The obligate requirement for serum in this assay presents difficulties in the direct