Progress Report for Contract Number N00014-93-C-0019. Quarterly Report Nomber 8.(Hawaii Biotechnology Group, Inc.)

摘要

1 The production of anti-ferret IgA antibodies. Status: Immunization of rabbitsand collection of sera completed. Mouse ascites successfully cross-adsorbed; material sent to NMRI researchers in 12/94. (2) The purification of lipopolysaccharide from Sbigella and Campylobacter species. Status: Shigella sonnei and S. flexneri LPS delivered to NMRI researchers. Purification of C. jejuni LPS reinstated. Have collected 173mg of required a 200mg LPS. Production of remainder in progress. (3) The development of an enzyme immunoassay for the detection of enteroaggregative Escherichia coli heat-stable toxin. Status: Initial recombinants provided by Navy unsatisfactory. Work suspended in 10/93 due to contract funding reduction. Suitable recombinants still not available. (4) The production of monoclonal antibodies against strain-specific antigenic epitopes on Campylobacter coli flagella. Status: Completed 3 fusions. No strain specific MAbs identified.