Protected Nanoparticles for an Oral Single-Dose Staphylococcal Enterotoxin Vaccine. Phase 1.

摘要

Immunization requires multiple injections of antigens at certain intervals to achieve the protective immune response. This immunization protocol is not practical in general especially for Army personnel in the field or in remote areas where access to the health care personnel may be difficult. Oral administration of vaccine is the most desirable route of immunization but often results in poor immune response, mostly because of the gastric degradation and inefficient localization of the vaccine to the gut-associated lymphoid tissue. Our objective in this project is to develop a single-dose oral vaccine system. In the Phase I SBIR, we have demonstrated a several fold efficient uptake of nanoparticles (about - 100 nm) compared to larger sized microparticles by the Peyer's patch tissue. In addition, the PORT system capsule technology was established and adapted for the gastric protection and selective delivery of The enclosed nanoparticles to the intestine for uptake by the Peyer's patch tissue. No other comparable system with these advantages exists. In addition, an ELISA has been established for anti-SEB antibody detection in our laboratory. Thus, a strong feasibility is demonstrated in The Phase I SBIR for designing a single- dose oral vaccine using nanoparticle-PORT System technology.