Delivery of Vaccines By Biodegradable Polymeric Microcapsules with Bioadherence Properties. Phase 1.

摘要

The objective of this program is the development of vaccines provide lasting immunity with a single administration. To accomplish this, biodegradable polymer microspheres are employed to provide release of the antigen at intervals which mimic conventional booster injections. The in vitro release characteristics of poly-DL-lactide-co-glyoilide microspheres were studied using high performance liquid chromatography (HPLc) using a water soluble dye and a commercially available tetanus toxoid C fragment (recombinant). A vaccine was then prepared using type A botulinum toxoid C fragment, and this was used to immunize a series of adult mice. Over a 3 month period, plasma samples were collected permitting the measurement ot antibody levels raised to the antigen using enzyme linked immunosorbent assay (ELISA). After 3 months, the immunized animals were supplied to the Army laboratories for an aerosol challenge.