RNASEH2A -- a Putative Non-Oncogene Addiction Gene Target and Marker for Radiosensitivity in High Risk Prostate Cancer.

摘要

We proposed that RNASEH2A represents a novel type of gene, up- regulated in lethal prostate cancer to prevent catastrophic genomic instability and cell death and thereby acting to make prostate cancers resistant to treatment with radiation therapy. The major findings to date include (1) independent validation of the associate of RNASEH2A with tumor grade. (2) Identification and sample preparation of RNA specimens with lethal potential for analysis of RNAseH2a expression.(3) Observation that RNASH2A expression does not independently predict lethal prostate cancer.(4) Observation that RNASH2A expression does predict radio-sensitivity and response to treatment in men who underwent radical prostatectomy and subsequently had post operative radiation. (5) Analysis of prostate cells lines for endogenous expression of RNASH2a (6) construction of plasmids that allow to over express RNAseH2a in prostate cancer cell lines to evaluate their impact on radiation sensitivity.