Dietary Refinements in a Sensitive Fish Liver Tumor Model

摘要

A purified diet has been developed and proven adequate for growth of the medaka(Oryzias latipes), a sensitive teleost species proven responsive to hepatocarcinogeni agents. The overall nutritional adequacy of the diet, a casein based ration, was evaluated and compared to three diets: commercially available flaked fish food; live 1newly hatched Artemia; and a combination of flake diet supplemented with Artemia. Survival, growth, reproductive success, general and liver histopathology, and selected hepatic enzyme activities were compared in medaka from first feeding through reproductive maturity. The PC-diet proved adequate in all of the above criteria. This diet provides a standardized, nutritionally adequate and consistent alternative :to undefined conventional diets and is less likely to contain the range of xenobiotic possible in whole live food. Studies in progress indicate the suitability of the diet for aquatic toxicity/carcinogenicity studies.