In Vivo Studies of Hematopoietic Growth Factors for Use in Military Personnel

摘要

The secondary structures and thermal denaturation/renaturation of recombinanthuman erythropoietins were compared using UV circular dichroism. Marked differences were observed between proteins expressed by different host cell lines, presumably due to dissimilar glycosylation patterns. A pharmacokinetic study of monomeric erythropoietin carried out in baboons demonstrated a very short plasma half-life of three hours. The proper formulation of the protein for these studies was established. Work on erythropoietin +dimers progressed, but high yields in each reaction were different to achieve. Knowledge of the differing glycosylation patterns will help improve these yields. Work on erythroid colony stimulation factor was resumed, and rodent bone marrow cultures were established.