MicroRNA Regulation of CD44+ Prostate Tumor Stem/Progenitor Cells and Prostate Cancer Development/Metastasis.

摘要

During the last year of the grant period, we carried out in vitro and in vivo work to further understand the role of miR-141 in regulating PCa stem/progenitor cells and PCa development. We validated the under-expression of miR-141 in the purified CD44+ populations from 21 primary patient PCa samples. We also confirmed that miR-141 is a negative regulator of EMT and suppresses migration and invasion of PCa cells. We observed that miR-141 promotes tumorigenicity of bulk PCa cells, but suppresses the stem cell properties of CD44+ PCa cells. By studying the role of several different miRNA in PCa cells, we have proved our original hypothesis, which is miRNA play important role in regulating prostate cancer stem cells and PCa development and different miRNAs regulate different aspects of tumor development and distinct CSC properties, and together, they coordinately control the tumor progression.