Intelligent Materials and Structures Based on Ordered Assemblies of DNA andProtein Incorporated within Electroactive Polymeric Systems

摘要

New ways in which biological macromolecules could be interfaced with conductingpolymers and other types of immobilization matrices were investigated. The object was to create new classes of signal transducing biomaterials that could be used in the design of intelligent materials incorporating: DNA; the photodynamic proteins: the phycobiliproteins and bacteriorhodopsin; and the enzymes alkaline phosphatase, horseradish peroxidase, laccase, glucose oxidase and alcohol oxidase. We have interfaced these biological macromolecules with: the conducting polymers polypyrrole and biotinylated alkylpolythiophenes of varying alkyl chain and biotin spacer lengths(using the classical biotinstreptavidin interaction), in Langmuir-Blodgett monolayers(our cassette' system), thin film and electrochemically synthesized thick films; optical fiber surfaces by hydrophobic polymer attachment and crosslinking to silanized surfaces; and through entrapment in the cavities of sol-gels during their polymerization process. We utilized these interaction and immobilization strategies to create monolayer thick phenolic and aniline based polymers on the LB trough surface by enzymatic polymerization. Proof of principle has been established for a second harmonic