Validation of the Mouse Ear Model as an In Vivo Bio-Assay for the Assessment ofTopical Mustard (HD) Injury

摘要

Topically applied sulfur mustard (HD) causes a delayed dermal inflammatoryresponse characterized by pronounced vesiculation in human skin. This inflammatory response may be due to increased vascular permeability (IVP) or actual blood vessel damage, resulting in extravasation of formed blood components. Inflammatory lesions characteristically include three features: hyperemia, increased vascular permeability of serum proteins, and leukocyte extravasation. The croton oil vesicant ear model of Tonelli is a well-developed assay procedure for the evaluation of the cutaneous effects of inflammatory agents. Lovetizetti found the croton oil induced ear edema assay to be a very useful method to detect topical activity of anti-inflammatory drugs. Several investigators quantitated the chemically induced inflammatory responses in the mouse ear by measuring radioactivity as counts per minute (CPM) of 1251-bovine serum albumin. Previous experiences in our laboratory, as well as a literature search and review of published information on mouse ear assays, have suggested that the mouse ear model may be applied to the problem of sulfur mustard injury. Compounds of interest to this study are niacinamide, a stimulator of NAD+