Prolonged Preservation of Human Platelets for Combat Casualty Care.

摘要

This report describes a method for the extended storage of human platelets. The platelet storage is performed at 40C to eliminate the bacterial contamination problems associated with conventional storage. To abrogate platelet storage lesions which occur during the 40C storage, the cells are treated with second messenger effectors which stabilize the cells by inhibiting the biochemical events which lead to storage lesions. Thus, following storage the cell remain active. Following 5 days of storage at 40C, platelets treated with this stabilizing solution display activity profiles as follows: 92% recovery of cell number; 45% ADP-induced aggregation; 80% collagen-induced aggregation; 61% hypotonic shock response; 10.5% extent of shape change, 74% expression of the surface marker GPIb; and 44% expression of the activation marker GMP-I40. After storage at 40C for 9 days the treated platelets yielded the following functional activity values: 91% recovery of cell number; 43% ADP- induced aggregation; 72% collagen-induced aggregation; 48% hypotonic shock response; 11.6% extent of shape change; 64% expression of iPib; and 50% expression of GMP-140. The results of this project demonstrate that the implementation of this method will allow platelets to be stored at 40C for extended periods without loss of activity.