Evaluation of Triple-Band Filters for Quantitative Epifluorescence Microscopy

摘要

The performance characteristics of two sets of triple-band epifluorescencefilters have been evaluated for use with digitally enhanced fluorescence microscopy. Use of such filters, at most, requires movement of the excitation filter, while the dichroic and emission filters remain fixed, allowing multi-wavelength imaging to be performed on standard microscopes. The dyes appropriate for use with these particular filters include Texas Red (TR), Bodipy (BD), FITC and Cascade.